Journal: Science Advances

Article Title: α-Synuclein expression is required for somatodendritic dopamine release and immediate early gene induction

doi: 10.1126/sciadv.ady6978

Figure Lengend Snippet: ( A ) Representative single-channel currents recorded in a cell-attached configuration from cultured WT and SKO SN DA neurons in the presence of N-, P/Q-, and R-type channel blockers show unitary currents (downward deflections) due to LTCC openings elicited by a voltage ramp (bottom trace). ( B ) Single-channel current amplitude was identical for LTCC in WT and SKO mice (ns by two-way ANOVA; n = 8 WT and 9 SKO). ( C ) Ensemble average P O - V relationships show no change in the probability of channel openings between WT and SKO neurons (ns by two-way ANOVA). ( D ) Population data confirm no change in maximal open channel probability (ns by t test). ( E and G ) Representative confocal images of cultured hippocampal neurons (14 days postplating) from WT and SKO mice immunostained for α1C (E) or α1D (G) subunits of the LTCC, as well as αSyn and pan-neuronal microtubule-associated protein 2 (MAP2). Note that staining intensity of the α1D subunit was low, making the quantification of the signal less reliable. Scale bar, 10 μm. ( F and H ) Analysis of average cytosolic and membrane α1C (F) and α1D (H) staining intensity ( n = 70 to 78 cells in each group from three independent experiments. * P < 0.05, ** P < 0.01, or **** P < 0.0001 by t test).

Article Snippet: Primary antibodies include anti-actin (mouse monoclonal, Sigma-Aldrich, #A5441, RRID: AB_476744; 1:1000), anti–glyceraldehyde-3-phosphate dehydrogenase (mouse monoclonal, Proteintech, #60004-1-Ig, RRID: AB_2107436; 1:1000), anti–pCREB (Ser 133 , rabbit monoclonal, Cell Signaling Technology, #9198S; 1:500), anti-CREB1 (rabbit polyclonal, ABclonal, #A11064, RRID: AB_2758389; 1:500), anti–c-Fos (rabbit monoclonal, Cell Signaling Technology, #2250S, RRID: AB_2247211; 1:1000), anti-Ca v 1.2 α1C (rabbit polyclonal, Proteintech, #21774-1-AP, RRID: AB_2878918; 1:500), and anti-Ca v 1.3 α1D (rabbit polyclonal, Alomone Labs, #ACC-005, RRID: AB_2039775; 1:100).

Techniques: Cell Culture, Staining, Membrane

Journal: bioRxiv

Article Title: CA inhibitor 1 treatment has only transient effects on estrous cyclicity in mice

doi: 10.64898/2026.01.12.699062

Figure Lengend Snippet: (A) CA inhibitor 1 administration resulted in a significant delay in the start of the next estrous cycle. (B) CA inhibitor 1 administration did not impact estrous cycle length across the 5 cycles. (C) CA inhibitor 1 did not impact average estrous cycle length. (D) CA inhibitor 1 significantly increased the proportion of normal length cycles compared to vehicle-treated mice. (E) CA inhibitor 1 did not impact ovarian weight.

Article Snippet: Adult female mice (n = 15) were subcutaneously injected with GS-6207 analog, CA inhibitor 1 (15mg/kg, MedChemExpress, Monmouth Junction NJ, HY-124594), a long-acting HIV capsid inhibitor, or 5% DMSO in sesame oil.

Techniques:

Journal: bioRxiv

Article Title: CA inhibitor 1 treatment has only transient effects on estrous cyclicity in mice

doi: 10.64898/2026.01.12.699062

Figure Lengend Snippet: (A) Schematics of location of mPOA. (B) Representative image of GFAP immunoreactivity in the mPOA of vehicle-treated mice. (C) Representative image of GFAP immunoreactivity in the mPOA of Ca inhibitor 1-treated mice. (D) CA inhibitor 1 did not impact GFAP immunoreactivity in the mPOA. (E) Representative image of ΔfosB immunoreactivity in the mPOA of vehicle-treated mice. (F) Representative image of ΔfosB immunoreactivity in the mPOA of CA inhibitor 1-treated mice. (G) CA inhibitor 1 did not impact ΔfosB immunoreactivity in the mPOA.

Article Snippet: Adult female mice (n = 15) were subcutaneously injected with GS-6207 analog, CA inhibitor 1 (15mg/kg, MedChemExpress, Monmouth Junction NJ, HY-124594), a long-acting HIV capsid inhibitor, or 5% DMSO in sesame oil.

Techniques: